Structural and Functional Studies on Component X of Mammalian Pyruvate Dehydrogenase Multienzyme Complex

Neagle, James Campbell (1990) Structural and Functional Studies on Component X of Mammalian Pyruvate Dehydrogenase Multienzyme Complex. PhD thesis, University of Glasgow.

Abstract

Pyruvate dehydrogenase is a multienzyme complex consisting of three enzymes, a decarboxylase (E1l), an acetyltransferase (E2) and a dehydrogenase (E3). A fourth enzyme of unknown function component X or protein X has recently been identified. Several similarities between E2 and protein X have been demonstrated. Further investigation into this unique polypeptide has shown monospecificity of antiserum raised against gel purified protein X and E2. In various cell lines, antiserum to protein X failed to recognise the E2 component, similarly with E2 antiserum no cross reactivity with protein X polypeptide was observed.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Biochemistry
• Date of Award: 1990
• Depositing User: Enlighten Team
• Unique ID: glathesis:1990-77042
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 09:22
• Last Modified: 14 Jan 2020 09:22
• URI: https://theses.gla.ac.uk/id/eprint/77042