Molecular Cloning and Analysis of Tomato Blackring Virus

Lochead, Susan Milne (1985) Molecular Cloning and Analysis of Tomato Blackring Virus. MSc(R) thesis, University of Glasgow.

Abstract

Tomato blackring virus RNA was reverse transcribed into single-strand complementary DNA. The RNA template was removed by alkaline hydrolysis, and the cDNA was transcribed into a double-stranded structure. The remaining single-stranded regions were removed by digestion with S1 nuclease and the recessed 3'-termini converted to a double-stranded structure. The cDNA was fractionated according to size.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Keywords: Plant sciences, Plant pathology
• Date of Award: 1985
• Depositing User: Enlighten Team
• Unique ID: glathesis:1985-77244
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 09:14
• Last Modified: 14 Jan 2020 09:14
• URI: https://theses.gla.ac.uk/id/eprint/77244