Identification and Structural Analysis of the Androgen Receptor From Normal and Androgen Insensitive Human Fibroblasts

McEwan, Iain Joseph (1987) Identification and Structural Analysis of the Androgen Receptor From Normal and Androgen Insensitive Human Fibroblasts. PhD thesis, University of Glasgow.

Abstract

Resistance to androgens in utero and at puberty causes a dysfunction of male sexual differentiation, and results in a form of male pseudohermaphroditism. From whole cell binding studies, using cultured human genital skin fibroblasts (GSF) a number of defects in the androgen receptor have been discovered, these have been classified as: absent, deficient, thermolabile, defective activation to the DNA/nuclear binding form, and finally, failure to "up-regulate" the basal binding level in response to prolonged (i.e. 24h) incubation of cells with hormone. There is, however, a need to study the receptor protein directly, without relying on the reversible binding of 3H ligand. Therefore, in the present study the androgen receptor from human GSF was extracted using 0.5M-KCl, and partially purified by 35% ammonium sulphate preciptitation prior to further studies.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Medicine
• Date of Award: 1987
• Depositing User: Enlighten Team
• Unique ID: glathesis:1987-77506
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77506