Studies on Immunity to Bovine Papillomaviruses

O'Neil, Brian William (1988) Studies on Immunity to Bovine Papillomaviruses. MSc(R) thesis, University of Glasgow.

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In the last few years there has been an intensive study of papillomavirus infections in both humans and animals. To date this has resulted in the recognition of 51 distinct types and several sub-types of human papillomaviruses (HPVs) and 7 types of bovine papillomaviruses (BPVs). This interest has stemmed from the fact that all these viruses are oncogenic and several are potentially carcinogenic in their natural hosts, which could possibly provide a valuable insight into the molecular mechanism of neoplasia. Cattle provide us with an excellent animal model system for papillomavirus infections in humans, as several manifestations are common in the two systems; multiple virus types, specificity of lesions, malignant progression of some papillomas and the involvement of cofactors. The studies described in this thesis record a series of experiments which help to detail the pathogenesis of BPVs and the subsequent immunity by the injection of purified virions. Chapter 1 reviews the features of naturally occurring HPV and animal papillomavirus infections. It details the various isolates, their physical properties, their replication cycle, their host range and their potential oncogenicity. A detailed description of the main papillomavirus infections in both the human and the bovine system are given. Chapter 2 describes the development of the techniques and reagents used in the subsequent experiments. The methodology provided the purified virus to; 1) experimentally induce BPV infections, 2) to localise BPV antigens and 3) to immunise against BPV infections. In Chapter 3 study of the detailed pathogenesis of papillomavirus infections caused by BPVs was possible by serially biopsying the experimentally transmitted tumours. This permitted the study of the tumour cycle; infection → tumour induction → tumour regression by BFV-2, 4, 5 & 6. Chapter 4 investigates the status of infection and reinfection among different papillomavirus types. Chapter 5 describes the various prophylactic vaccination agents used in order to assess their ability to induce rejection of a papillomavirus infection. The attempt to show protection by immunising with bovine cells transformed in vitro failed. However it was demonstrated that cattle could be protected against homologous infection and oncogenesis by vaccines prepared from virus and tumour extracts. There was no obvious advantage in administering virus along with the cellular suspension. Prophylactic vaccination using purified virions from BPV-2, 4 and 6 was very successful and it also showed that this was a type-specific immunity. (Abstract shortened by ProQuest.).

Item Type: Thesis (MSc(R))
Qualification Level: Masters
Keywords: Veterinary science
Date of Award: 1988
Depositing User: Enlighten Team
Unique ID: glathesis:1988-77691
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jan 2020 11:53
Last Modified: 14 Jan 2020 11:53

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