In Vitro and In Vivo Ocular Studies Using Herpes Simplex Virus Types 1 and 2

Cook, Stuart Douglas (1988) In Vitro and In Vivo Ocular Studies Using Herpes Simplex Virus Types 1 and 2. PhD thesis, University of Glasgow.

Abstract

The biological properties of three HSV strains were characterized with reference to ocular disease in the rabbit. Two HSV-1 strains, 17 and McKrae, and the HSV-2 strain HG52 were studied and the following parameters were assessed; clinical disease; virulence; spontaneous shedding of HSV; induced shedding of HSV; neural latency; and corneal latency. Intratypic and intertypic differences were apparent. The HSV-l strain 17 was pathogenic to rabbit eyes and neuropathogenic with increasing titres of inoculum. It had a low frequency of spontaneous shedding and an intermediate frequency of induced shedding. The HSV-1 strain 17 was able to establish latent infections within trigeminal ganglia. The HSV-1 strain McKrae was pathogenic to rabbit eyes and particularly neuropathogenic. It had a high frequency of both spontaneous and induced viral shedding. The McKrae strain was able to establish latent infections within trigeminal ganglia but differed in maintaining a latent infection within the cornea. The HSV-2 strain HG52 was non-pathogenic to rabbit eyes and non-neuropathogenic. It had a very low frequency of spontaneous and induced shedding. The HSV-2 strain HG5 2 was able to maintain latent infections within the trigeminal ganglion. Twelve corneas from patients suffering from herpes simplex keratitis were collected and analysed by light microscopy, electron microscopy and organ culture. Two of the twelve corneas released HSV after at least seven days in organ culture. The released virus was identified as HSV-1 by restriction endonuclease analysis. Primary cultures of rabbit corneal epithelial cells, keratocytes and endothelial cells were established. The identity of the cells was confirmed by electron microscopy and indirect immunofluorescence techniques. The one step growth kinetics of HSV-1 in the three distinct cell types were established. Latent infections were established in the distinct cell lines in vitro using supra optimal temperatures. Cellular stress proteins were demonstrated at supraoptimal temperatures. The antiviral agent acycloguanosine was unable to eliminate latent HSV infections at the supraoptimal temperature (42

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Virology, Ophthalmology
• Date of Award: 1988
• Depositing User: Enlighten Team
• Unique ID: glathesis:1988-77713
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77713