Cell Proliferation in Human Oral Mucosa

Warnakulasuriya, Kasturi Arachchi Ariya Saman (1976) Cell Proliferation in Human Oral Mucosa. PhD thesis, University of Glasgow.

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Studies of cell proliferation and migration - cell kinetics - have been described extensively on animal tissues and to a more limited extent in human tissues. These reported studies are reviewed and the deficiencies in available knowledge are highlighted. One of the main difficulties in studying the kinetics of human tissues is that the most widely used technique in animal work, namely parenteral injection of radioactive isotopes to label specific cells, is not generally applicable in man. The initial work in the present study was concerned with investigation of techniques for quantitating cell kinetics and the development of an in vitro radioactive labelling technique suitable for use with human tissues. The reliability of this technique was evaluated in the animal study where the in vitro labelling was shown to give comparable results to the classical in vivo labelling techniques. The in vitro radioactive labelling technique was then used on biopsies from normal cheek mucosa obtained at six times of the day. This allowed diurnal variations in cell kinetic parameters to be quantitated. In conjunction with this investigation a stereologic study of the epithelium was undertaken. The cell kinetic and stereologic techniques were next used to study eight cases of leukoplakia of cheek, a clinical disorder characterised by keratosis. The findings in the leukoplakia lesions were contrasted with those in biopsies from clinically normal mucosa in the same individuals. Epithelial cell production was found to be increased although the viable cellular layers of the epithelium were atrophic in the leukoplakias. The control biopsies, although obtained from clinically normal mucosa, were shown to exhibit several abnormalities. The degree of increased cell producation in leukoplakia in these control specimens was shown to have a positive correlation with the severity of epithelial atypia. The further applications of kinetic studies and epithelial morphometry are outlined and the difficulties encountered in the use of in vitro radioactive labelling techniques and other morphometric methods in the study of cell proliferation are discussed.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Dentistry, Pathology, Cellular biology
Date of Award: 1976
Depositing User: Enlighten Team
Unique ID: glathesis:1976-78713
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 30 Jan 2020 14:59
Last Modified: 30 Jan 2020 14:59
URI: https://theses.gla.ac.uk/id/eprint/78713

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