The Experimental Production of Metaplasia and Hyperplasia in the Serosal Endothelium of the Visceral Pleura and in the Alveolar Epithelium of the Lung of the Rabbit. Appendix A: Lesions of the Liver and Other Organs Produced by the Intrapleural Injection of Sudan III. Appendix B: The incidence of Spontaneous Tumours and Certain Other Lesions in a Consecutive Series of 700 Rabbits

Young, John Stirling (1929) The Experimental Production of Metaplasia and Hyperplasia in the Serosal Endothelium of the Visceral Pleura and in the Alveolar Epithelium of the Lung of the Rabbit. Appendix A: Lesions of the Liver and Other Organs Produced by the Intrapleural Injection of Sudan III. Appendix B: The incidence of Spontaneous Tumours and Certain Other Lesions in a Consecutive Series of 700 Rabbits. MD thesis, University of Glasgow.

Abstract

Certain methods are described, based upon the intrapleural injection in the rabbit of certain biological reagents, whereby hyperplasia and metaplasia of the serosal endothelium, covering the visceral pleura, and of the alveolar epithelium of the lung can be produced. (1) a. Sudan lII, dissolved in olive oil and emulsified by sodium chelate (bile salts) induces a transformation of the seros endothelium into columnar, transitional, or stratified squamous epithelium, while the pulmonary epithelium lining the marginal alveoli of the lung undergoes metaplasia to a tall columnar type. These changes are generally maintained for two or three months but they have been witnessed in two exceptional animals after sixteen months. The dye is absorbed from the pleural sac very slowly and incompletely and the residue remains in contact with the surface of the lung for an indefinitely long period until it becomes encapsulated by fibrous tissue. b. sudan III, dissolved in olive oil but not emulsified by bile salts, is relatively ineffective. (2) Sodium cholate, whether emulsified in olive oil or in liquid paraffin or dissolved in physiological saline, produces wellmarked hyperplasia and metaplasia of the epithelium lining the marginal alveoli of the lung. These changes are transient, attaining a maximum within four days and undergoing involution within eight days. (3) Certain solutions of various electrolytes produce reactions which conform in their histological characters and in their evolution with the reaction determined by bile salts. The hyperplasia increases as the valency of the cation of the salt increases, but it is not enhanced by increasing the valency of the anion. It has not been possible to demonstrate any antagonism between sodium and calcium ions, or potassium and calcium ions,in respect of the production of epithelial proliferation when they are exhibited in chemically equivalent proportions (3/8 N solutions). (4) Minor degrees of the same type of epithelial hyperplasia are produced by the intrapleural injection of large volumes of distilled water. (5) "Hypertonic" solutions (3/4 M - 10 c.c.) of two nonelectrolytes - cane-sugar and urea- produce no reaction. (6) It is not possible to sustain the transient reaction produced by 5 c.c. of a 3/4 N solution of strontium chloride by repeating the injection at intervals of 48 hours. On the contrary, there is some reason to believe that the proliferating cells arising from the first injection become "refractory" to subsequent injections of the same solution. (7) The significance of physical factors in the genesis of the epithelial proliferation is discussed and attention is directed towards certain observations which may affect the maintenance or the stability of the reaction.

• Item Type: Thesis (MD)
• Qualification Level: Doctoral
• Keywords: Medicine, Pathology
• Date of Award: 1929
• Depositing User: Enlighten Team
• Unique ID: glathesis:1929-79835
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 03 Mar 2020 10:32
• Last Modified: 03 Mar 2020 10:32
• URI: https://theses.gla.ac.uk/id/eprint/79835