Manipulation of mesenchymal stem cell differentiation via gold nanoparticle-mediated delivery of antagomiRs

Mathew, Shijoy (2020) Manipulation of mesenchymal stem cell differentiation via gold nanoparticle-mediated delivery of antagomiRs. PhD thesis, University of Glasgow.

Abstract

The human body is a complex piece of organic machinery, and as such, can be prone to faults and deterioration. There are many medical conditions that can be attributed to bone or cartilage deterioration and damage, like osteoarthritis and osteoporosis. Regeneration of these tissues is an on-going challenge, which have been targeted in many different ways.
MicroRNAs are short sequenced RNAs that are involved in the regulation of nearly 60 percent of the genes in the body. This project aims to exploit their function to promote differentiation into bone, fat or cartilage cells. This is achieved using nanoparticles as the delivery platform. The use of nanoparticles in medicine has picked up at a rapid rate, and its small size, non-toxicity and multi-valancy allows for a safer and customizable method of cargo delivery. A novel form of inducing osteogenesis is the using of nanovibrations, known as nanokicking.
This project aims to use antagomirs or anti-microRNAs as the primary cargo, which is a nucleotide sequence that can bind to the microRNA, causing a loss of function. A combination of nanokicking and functionalised GNP treatment will be used to see if there is an improvement in the efficacy and time taken for differentiation.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Mesenchymal Stem Cells, MicroRNAs, Gold Nanoparticles, miRNA, MSCs, Differentiation, Nanokicking, Nanovibrations
• Colleges/Schools: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Supervisor's Name: Berry, Dr. Catherine and Dalby, Prof. Matthew
• Date of Award: 2020
• Depositing User: Mr. Shijoy Mathew
• Unique ID: glathesis:2020-81568
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 07 Aug 2020 09:15
• Last Modified: 12 Sep 2022 08:51
• URI: https://theses.gla.ac.uk/id/eprint/81568