Parr, Yasmin Amy (2020) Investigating the biology underlying feline leukaemia virus diagnostics to help predict the pathogenesis of infection. PhD thesis, University of Glasgow.
Due to Embargo and/or Third Party Copyright restrictions, this thesis is not available in this service.Abstract
Feline leukaemia virus (FeLV) infects domestic cats and other wild felids worldwide. FeLV exposure has three potential outcomes, namely abortive, regressive and progressive infection. The early interactions between FeLV and the haematologic and lymphatic systems of the host determine the pathogenesis of infection and the immune response appears to have a major influence on disease progression. The aim of this thesis was to investigate current diagnostic tests for FeLV infection as well as the humoral immune response of cats naturally exposed to FeLV and to determine whether an assay could be developed that would predict the outcome of infection. A total of 489 samples from 432 domestic cats, from several cohorts, were investigated, as well as 113
samples from 112 non-domestic exotic feline species. Firstly, the development and optimisation of three study specific methods is described. The first assay to be developed was a novel enzyme-linked immunosorbent assay (ELISA) to detect antibodies recognising FeLV-A and FeLV-B surface unit (SU) glycoproteins in cat plasma. Secondly, a product enhanced reverse transcriptase (PERT) assay to detect reverse transcriptase in culture fluids from in vitro peripheral blood mononuclear cell (PBMC) cultures was optimised. Thirdly, a quantitative polymerase chain reaction (qPCR) assay was developed to simultaneously detect FeLV provirus and the housekeeping gene control feline
| Item Type: | Thesis (PhD) |
|---|---|
| Qualification Level: | Doctoral |
| Keywords: | Feline leukaemia virus, FeLV, veterinary virus, veterinary diagnostics, retrovirus, feline virology. |
| Subjects: | Q Science > QR Microbiology Q Science > QR Microbiology > QR180 Immunology Q Science > QR Microbiology > QR355 Virology |
| Colleges/Schools: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Funder's Name: | IDEXX Laboratories |
| Supervisor's Name: | Hosie, Professor Margaret |
| Date of Award: | 2020 |
| Embargo Date: | 7 March 2021 |
| Depositing User: | Dr Yasmin Parr |
| Unique ID: | glathesis:2020-81640 |
| Copyright: | Copyright of this thesis is held by the author. |
| Date Deposited: | 08 Sep 2020 11:19 |
| Last Modified: | 01 Aug 2022 08:43 |
| URI: | https://theses.gla.ac.uk/id/eprint/81640 |
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