Bioinformatic and biochemical characterization of arabidopsis thaliana protein nch1 and links to plant immunity

Salmirinne, Irni Onerva (2022) Bioinformatic and biochemical characterization of arabidopsis thaliana protein nch1 and links to plant immunity. MSc(R) thesis, University of Glasgow.

Abstract

NCH1 (NRL PROTEIN FOR CHLOROPLAST MOVEMENT 1) is a plant protein postulated to play a role in plant immunity, in addition to being a component of the plant blue light signalling pathway directly downstream of the phototropin blue light receptors. Confocal microscopy of mCitrine-tagged NCH1 Arabidopsis lines and bifluorescence complementation (BiFC) experiments in Nicotiana benthamiana suggest that in its native state, Arabidopsis protein AtNCH1 exists as a dimer, therefore confirming the similar results reported previously in its potato ortholog StNRL1. AtNCH1 was also found to localize at the plasma membrane both in the dark and after blue light treatment both in transgenic Arabidopsis hypocotyls and in the transient Nicotiana benthamiana expression system. Preliminary confocal microscopy studies on the putative phosphorylation-deficient variant, NCH1S602A-S604A, also exhibited similar localization in the plasma membrane in N. benthamiana. As it was hypothesised that NCH1 could play a role in Arabidopsis immunity against the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), flood assay data suggested that double mutant nch1-1/rpt2-3 plants showed inconclusive differences in impairment in immunity against Pst DC3000 in comparison to the wild type Col-0 plants. Further perspectives to the role of NCH1 in plant immunity include the determination of the phosphorylation state, and the roles of potential NCH1 interacting partners, including potential hetero interactions with NRL family proteins, on Arabidopsis immunity.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Colleges/Schools: College of Medical Veterinary and Life Sciences
• Supervisor's Name: Jenkins, Prof. Gareth
• Date of Award: 2022
• Depositing User: Theses Team
• Unique ID: glathesis:2022-82852
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 06 May 2022 08:58
• Last Modified: 06 May 2022 08:59
• Thesis DOI: 10.5525/gla.thesis.82852
• URI: https://theses.gla.ac.uk/id/eprint/82852