Transcriptomic analysis of canine B and T cell lymphoma

Lopes, Mariana Guedes (2024) Transcriptomic analysis of canine B and T cell lymphoma. MVM(R) thesis, University of Glasgow.

Abstract

Gene expression profiling in human lymphoma has identified a number of genes and pathways of prognostic value that could also be used as targets for therapy.

The aim of the present study was to compare gene expression in canine B-cell lymphoma, T-cell lymphoma and control lymph node samples using RNA-Seq to identify differentially expressed pathways which may have a role in pathogenesis and could be used as possible treatment targets.

RNA was extracted from lymph node tissue collected from six dogs with B-cell lymphoma, five dogs with T-cell lymphoma, one dog with B and T-cell lymphoma and eight controls. The RNA was sequenced using an Illumina NextSeq500 platform, reads were aligned to the current canine genome and gene expression profiles were determined for each group. Pathway analysis was also performed to identify networks enriched for differentially expressed genes together with activated and repressed molecular pathways. After stand-alone analysis of the dataset, it was combined with a previous dataset representing eighteen dogs with B-cell lymphoma and five dogs with T-cell lymphoma in order to increase the power of the analysis.

Principal component analysis revealed that the three groups, i.e. B-cell lymphoma, T-cell lymphoma and controls, formed separate clusters with distinct expression signatures. Network analysis highlighted different pathways overrepresented in each type of lymphoma. In B-cell lymphoma tissue, the NF-ĸB pathway, cell cycle control and DNA repair pathways were over-represented with overexpression of ribosomal and minichromosome maintenance proteins also noted. In contrast for T-cell lymphomas, mRNA synthesis, FGFR and RUNX pathways were highlighted together with those relating to innate immunity.

This project identified several aberrant pathways in B-cell and T-cell lymphoma that have not previously been reported and which can be considered candidate targets for developing novel treatment, thereby informing future studies.

• Item Type: Thesis (MVM(R))
• Qualification Level: Masters
• Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer); S Agriculture > SF Animal culture > SF600 Veterinary Medicine
• Colleges/Schools: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Supervisor's Name: Morris, Professor Joanna, Weir, Professor Willie, Waugh, Elspeth and Capewell, Dr. Paul
• Date of Award: 2024
• Depositing User: Theses Team
• Unique ID: glathesis:2024-84060
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 05 Feb 2024 16:28
• Last Modified: 05 Feb 2024 16:31
• Thesis DOI: 10.5525/gla.thesis.84060
• URI: https://theses.gla.ac.uk/id/eprint/84060