Application of disposable chiral plasmonics for biosensing and Raman spectroscopy

Kartau, Martin (2024) Application of disposable chiral plasmonics for biosensing and Raman spectroscopy. PhD thesis, University of Glasgow.

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This thesis explores the capabilities of disposable chiral plasmonic metafilm assays, termed Disposable Plasmonic Assays, as a promising platform for biosensing and surface-enhanced Raman spectroscopy. The sensing and Raman properties of these metafilms arise from the excitation of surface plasmons when exposed to incident light. These plasmonic properties strongly depend on the geometric characteristics of the constituent nanostructures found in the metafilms. Specifically, the primary nanostructure employed throughout this research is the chiral 'shuriken' star, which generates chiral electromagnetic fields exhibiting greater chiral asymmetry than circularly polarized light.

Monitoring changes in the resonance positions of the characteristic optical rotatory dispersion spectra produced by the Disposable Plasmonic Assays allows for the observation of surface binding events. By measuring resonance shift data and through the utilisation of various gold film functionalisation techniques, these assays are demonstrated as versatile, label-free biosensing platforms capable of specifically detecting a wide range of target proteins and virus particles from complex solutions. Furthermore, the multiplexing performance of these assays is showcased, enabling the detection of multiple different antigens and virions in a single experiment. These results highlight the potential of plasmonic metafilms as rapid and disposable point-of-care immunoassays for diagnostic applications.

In addition to biosensing, the chiral geometry of Disposable Plasmonic Assays is exploited for the chiral discrimination of metal nanoparticles and small molecules using Surface Enhanced Raman Spectroscopy (SERS). By linking helicoid shaped gold nanoparticles to the metafilm surface via a dithiol linker, the chiral properties of both nanoparticles and metafilms combine, resulting in the creation of differential electromagnetic 'hotspot' regions based on their symmetry combinations. The electromagnetic intensity in these regions corresponds to the SERS signal obtained from the achiral dithiol linker molecule, facilitating a deeper understanding of the chirally dependent SERS phenomenon. These findings serve to validate and explain the differential SERS data obtained enantiomers of biomolecules and drug molecules from silver modified Disposable Plasmonic Assays.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Subjects: Q Science > QD Chemistry
Colleges/Schools: College of Science and Engineering > School of Chemistry
Supervisor's Name: Karimullah, Dr. Affar
Date of Award: 2024
Depositing User: Theses Team
Unique ID: glathesis:2024-84066
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 06 Feb 2024 13:36
Last Modified: 06 Feb 2024 13:38
Thesis DOI: 10.5525/gla.thesis.84066
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