A study of salt tolerance in Arabidopsis thaliana and Hordeum vulgare

Attumi, Alarbe (2007) A study of salt tolerance in Arabidopsis thaliana and Hordeum vulgare. PhD thesis, University of Glasgow.

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ABSTRACT The original objective of this work was to compare the cellular processes in salt tolerant and salt sensitive plants cells to gain insight into the mechanisms that confer halotolerance. Halotolerant and salt sensitive cell lines were derived from the model glycophyte Arabidopsis thaliana; in addition cell suspension cultures from the dicot halophytes Beta vulgaris and Atriplex halimus were also generated. Unfortunately, severe disruptions were encountered following a serious fire; persistent power failures, and failures of new equipment hampered progress with this work. For this reason, only comparisons between the Arabidopsis cell lines were completed. The halotolerant (HHS) cell lines survival strategy is to prevent Na accumulation when grown in < 100 mM NaCl. Wild type (WT) cells grow faster than HHS cells in the absence of NaCl, but rapidly take up Na in 50 mM NaCl where their growth is severely affected, and fail to grow completely above 100 mM NaCl. No evidence was found to suggest this growth impairment arose from osmotic stress or nutrient ion deficiencies. Protein profiling of HHS cells identified a number of proteins whose abundance is regulated by salt stress. These included proteins involved in ion transport, central metabolism, and general stress responses. The implications of these findings are discussed. In a separate project, a whole plant approach was taken to establish the physiological mechanisms that account for the reported difference in halotolerance between two commercially grown barley lines originating from China. Measurements on growth and development, plant water status, tissue ion profiling, photosynthesis rates, and transpiration rates suggested the tolerant line (Zhou 1) enters the reproductive phase of its life cycle approximately one week earlier than the sensitive line (Zhou 85), and this critical period allows floral development resulting in improved yields. This early ii flowering is not associated with the well characterized PpD-H1 locus controlling early flowering in cereals. The main conclusion from this study is that for glycophytes that do not complete a full life cycle above 100 mM NaCl (which includes all of the world’s major crops), it is the ionic component of salinity stress that impairs growth and yield. Further research on salinity stress in crops should focus on understanding the processes that control ionic balance rather than osmoregulation.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Salt tolerance, Arabidopsis thaliana, Hordeum vulgare.
Subjects: Q Science > QK Botany
Colleges/Schools: College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Funder's Name: UNSPECIFIED
Supervisor's Name: Dominy, Dr. Peter
Date of Award: 2007
Depositing User: Mr Alarbe Attumi
Unique ID: glathesis:2007-114
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 26 Feb 2008
Last Modified: 24 Apr 2018 15:44
URI: http://theses.gla.ac.uk/id/eprint/114

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