Synthetic biology in droplet-based microfluidics

Mohammadi, Kimia (2016) Synthetic biology in droplet-based microfluidics. PhD thesis, University of Glasgow.

Due to Embargo and/or Third Party Copyright restrictions, this thesis is not available in this service.

Abstract

Droplet microfluidics is an active multidisciplinary area of research that evolved out of the larger field of microfluidics. It enables the user to handle, process and manipulate micrometer-sized emulsion droplets on a micro- fabricated platform. The capability to carry out a large number of individual experiments per unit time makes the droplet microfluidic technology an ideal high-throughput platform for analysis of biological and biochemical samples. The objective of this thesis was to use such a technology for designing systems with novel implications in the newly emerging field of synthetic biology. Chapter 4, the first results chapter, introduces a novel method of droplet coalescence using a flow-focusing capillary device. In Chapter 5, the development of a microfluidic platform for the fabrication of a cell-free micro-environment for site-specific gene manipulation and protein expression is described. Furthermore, a novel fluorescent reporter system which functions both in vivo and in vitro is introduced in this chapter. Chapter 6 covers the microfluidic fabrication of polymeric vesicles from poly(2-methyloxazoline-b-dimethylsiloxane-b-2-methyloxazoline) tri-block copolymer. The polymersome made from this polymer was used in the next Chapter for the study of a chimeric membrane protein called mRFP1-EstA∗. In Chapter 7, the application of microfluidics for the fabrication of synthetic biological membranes to recreate artificial cell- like chassis structures for reconstitution of a membrane-anchored protein is described.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Droplet-based microfluidics, synthetic biology, site-specific recombination, polymersome, protocell.
Subjects: Q Science > Q Science (General)
Q Science > QC Physics
Colleges/Schools: College of Science and Engineering > School of Engineering
Funder's Name: UNSPECIFIED
Supervisor's Name: Cooper, Professor Jonathan
Date of Award: 2016
Embargo Date: 22 August 2019
Depositing User: Ms Kimia Mohammadi
Unique ID: glathesis:2016-7596
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 27 Sep 2016 09:02
Last Modified: 20 Oct 2016 11:39
URI: http://theses.gla.ac.uk/id/eprint/7596

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