Studies of Vitamin A in Nutrition and Cancer

Al-Saleh, Maysoon Ahmed Saad (1986) Studies of Vitamin A in Nutrition and Cancer. PhD thesis, University of Glasgow.

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Abstract

This thesis describes the development of specific assays for separation and extraction of vitamin A (retinol) and its metabolites, retinal, retinoic acid, retinol acetate, retinol palmitate and its pro-vitamin carotene. The assay employed a liquid/liquid extraction procedure which gave reproducible recoveries for retinol and its metabolites. This was followed by a high pressure liquid chromatography (HPLC) procedure using a silica column with hexane:tetrahydrofuran:formic acid as mobile phase. This gave excellent chromatographic efficiency and resolution of metabolites and resulted in a highly sensitive and specific assay for all the compounds analysed. The assay was used for serum, and was modified to be applicable also for liver and nutritional solutions. A major benefit of this assay compared with previous methods is that all retinol metabolites can be separated on a single column without requirement for expensive gradient systems.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Nutrition
Date of Award: 1986
Depositing User: Enlighten Team
Unique ID: glathesis:1986-77247
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 14 Jan 2020 11:53
Last Modified: 14 Jan 2020 11:53
URI: http://theses.gla.ac.uk/id/eprint/77247

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