Studies of Vitamin A in Nutrition and Cancer

Al-Saleh, Maysoon Ahmed Saad (1986) Studies of Vitamin A in Nutrition and Cancer. PhD thesis, University of Glasgow.

Abstract

This thesis describes the development of specific assays for separation and extraction of vitamin A (retinol) and its metabolites, retinal, retinoic acid, retinol acetate, retinol palmitate and its pro-vitamin carotene. The assay employed a liquid/liquid extraction procedure which gave reproducible recoveries for retinol and its metabolites. This was followed by a high pressure liquid chromatography (HPLC) procedure using a silica column with hexane:tetrahydrofuran:formic acid as mobile phase. This gave excellent chromatographic efficiency and resolution of metabolites and resulted in a highly sensitive and specific assay for all the compounds analysed. The assay was used for serum, and was modified to be applicable also for liver and nutritional solutions. A major benefit of this assay compared with previous methods is that all retinol metabolites can be separated on a single column without requirement for expensive gradient systems.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Nutrition
• Date of Award: 1986
• Depositing User: Enlighten Team
• Unique ID: glathesis:1986-77247
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77247