Proteomic analysis of the mouse mammary gland

Davies, Claire Rebecca (2004) Proteomic analysis of the mouse mammary gland. PhD thesis, University of Glasgow.

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The mouse mammary gland develops mainly postnatally by highly regulated phases of invasive growth, ductal branching, differentiation, apoptosis and tissue remodelling during each pregnancy cycle. Detailed analyses of these stages of development were performed using proteomics in order to identify proteins that are regulated in these stages of development. Sixteen time points spanning the key stages of development were analysed and data mined by 2-D gel electrophoresis. Digital copies of the proteome images revealed the expression profiles of all the protein features detected across the developmental time points. These expression profiles were interrogated by creating two databases, and from these databases 125 protein features were selected for mass spectrometry analysis. Selection was based on the following criteria; features that were exclusively present in particular phases of development, and features that were altered during the switch from lactation to involution. Fifty nine protein identifications were determined by mass spectrometry. In addition to analysing these stages of development, specialised structures which drive ductal morphogenesis, the terminal end buds, were isolated and analysed by 2-D gel electrophoresis. Forty four protein features which showed differential expression between the terminal end buds and the adult virgin gland were chosen for mass spectrometry analysis. Of these protein features, 24 produced protein identifications. Detailed analyses were perfomied on selected protein identifications using western blot, immunohistochemistry, real-time RT-PCR and data available from a microarray study. Investigations on one particular protein identification, annexin A2, were extended to breast cancer tissue samples. Not all the protein features selected for mass spectrometry analysis revealed protein identifications. The expression profiles of these features were assessed again and 28 were chosen for reanalysis by mass spectrometry. The data generated for one particular protein identification, BTF3, indicates a possible regulatory role in mouse mammary gland development. This study has used proteomics as a tool for identifying proteins which regulate mouse mammary gland development. Many proteins were identified, and the follow-up studies performed highlighted a few as being important in this area of research.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Developmental biology.
Colleges/Schools: College of Medical Veterinary and Life Sciences
Supervisor's Name: Gusterson, Prof. Barry and Page, Dr. Martin
Date of Award: 2004
Depositing User: Enlighten Team
Unique ID: glathesis:2004-72550
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 11 Jun 2019 11:06
Last Modified: 23 Jul 2021 15:43

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