Crystallographic Studies on Macrophage Inflammatory Protein-1alph

MacLean, John K. F (1997) Crystallographic Studies on Macrophage Inflammatory Protein-1alph. PhD thesis, University of Glasgow.

Abstract

Macrophage Inflammatory Protein-1alpha is a member of the Chemokine family of inflammatory mediators. In addition to its chemotactic properties, MIP-1alpha is an inhibitor of the proliferation of primitive stem cells, and is therefore potentially useful in cancer therapy. It has also recently been shown to protect certain subsets of human cells from infiltration by HIV-1 virus. The crystal structures of three structural mutants of the Chemokine Macrophage Inflammatory Protein-la have been determined. The structure of a monomeric mutant has been determined to 2.9A, that of a dimeric mutant to 2.3A, and the structure of a tetrameric mutant has been determined in two crystal forms at 2.05A and 1.65A. This thesis outlines the process by which these mutant structures were solved and compares them with other Chemokine structures. The potential implications of the structures of the MIP-1alpha mutants are investigated. The complex network of Chemokines and Chemokine receptors is described, and attempts are made to rationalise receptor-binding specificities of MIP-1alpha and related proteins on the basis of the MIP-1alpha structures.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Additional Information: Adviser: Paul Ermsley
• Keywords: Biochemistry
• Date of Award: 1997
• Depositing User: Enlighten Team
• Unique ID: glathesis:1997-75276
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 19 Nov 2019 21:22
• Last Modified: 19 Nov 2019 21:22
• URI: https://theses.gla.ac.uk/id/eprint/75276