ISG expression screening reveals the family-specific antibunyaviral activity of ISG20

Feng, Junjie (2017) ISG expression screening reveals the family-specific antibunyaviral activity of ISG20. PhD thesis, University of Glasgow.

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Printed Thesis Information:


Order Bunyavirales poses a significant threat to the agricultural industry, animal husbandry and human welfare. In response to viral infections, interferons (IFNs) trigger the expression of numerous interferon stimulated genes (ISGs) whose concerted action can potently inhibit the replication of bunyaviruses. We used a FACS-based method to screen the ability of ~500 unique ISGs from humans or rhesus macaques to inhibit the replication of Bunyamwera virus (BUNV), the prototype of both the Peribunyaviridae family and Bunyavirales order. Candidates possessing antibunyaviral activity were further examined using a panel of divergent bunyaviruses in family Peribunyaviridae, Hantaviridae, Nairoviridae and Phenuiviridae.

Item Type: Thesis (PhD)
Qualification Level: Doctoral
Keywords: Bunyaviruses, innate immunity, ISGs screen, ribonuclease, in vitro evolution.
Subjects: Q Science > QR Microbiology > QR355 Virology
Colleges/Schools: College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Funder's Name: Wellcome Trust (WELLCOTR), Medical Research Council (MRC), Medical Research Council (MRC)
Supervisor's Name: Wilson, Dr. Sam and Shi, Dr. Xiaohong and Elliott, Prof. Richard
Date of Award: 2017
Embargo Date: 29 November 2020
Depositing User: Mr Junjie Feng
Unique ID: glathesis:2017-8610
Copyright: Copyright of this thesis is held by the author.
Date Deposited: 05 Dec 2017 13:47
Last Modified: 04 Dec 2019 16:19

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