Differential actions of methlyarginines in the rat aorta

Al-Zobaidy, Mohammed Abdul-Hassan Jabarah (2012) Differential actions of methlyarginines in the rat aorta. PhD thesis, University of Glasgow.

Abstract

The PhD project is about the Regulation of vascular tone by endothelium. It involves studying the effects of methylated arginine analogues such as monomethyl arginine (L-NMMA) and asymmetric dimethylarginine (ADMA) on nitric oxide (NO) activity in rat aorta using organ baths containing Krebs solution at 37 ˚C and gassed with 95% O2 and 5% CO2. These two inhibitors of endothelial nitric oxide synthase enzyme (eNOS) showed an anomalous action, as they inhibited basal NO activity (assessed by enhancement of phenylephrine-induced tone and by blocking relaxations produced by superoxide dismutase or the PDE5 inhibitor; T0156) but not that is stimulated by agonists like acetylcholine or the calcium ionophore A23187. After establishing these findings I will try to find the reason(s) for these paradoxical actions of these two endogenously synthesized inhibitors of eNOS.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Nitric oxide, Monomethylarginine (L-NMMA), asymmetric dimethylarginine (ADMA).
• Subjects: R Medicine > RM Therapeutics. Pharmacology
• Colleges/Schools: College of Medical Veterinary and Life Sciences > School of Life Sciences
• Supervisor's Name: Martin, Prof. William
• Date of Award: 2012
• Depositing User: Dr MOHAMMED AL-ZOBAIDY
• Unique ID: glathesis:2012-3838
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 16 Jan 2013 15:24
• Last Modified: 16 Jan 2013 15:24
• URI: https://theses.gla.ac.uk/id/eprint/3838