Synaptic interactions between cholinergic and GABAaergic systems of the hippocampus

Ferrigan, Leanne M. (2004) Synaptic interactions between cholinergic and GABAaergic systems of the hippocampus. PhD thesis, University of Glasgow.

Abstract

Gamma-aminobutyric acid (GABA) and acetylcholine (ACh) are important regulators of hippocampal function. The hippocampus is governed by a diverse population of GABAergic local circuit neurones (interneurones) and moreover, receives a dual cholinergic and GABAergic input from the medial septal nucleus (MSN). This thesis describes electrophysiological, pharmacological and neuroanatomical approaches in rodent brain slice preparations to investigate the interaction between these cholinergic and GABAergic systems of the mammalian hippocampus. Intracellular and patch clamp recordings were made from hippocampal pyramidal neurones in hippocampal and septo-hippocampal slice preparations. In the presence of ionotrophic glutamate and GABA receptor antagonists, electrical stimulation of afferent fibres within either the stratum oriens or MSN evoked an isolated slow excitatory postsynaptic potential (EPSP), with mean peak amplitudes of 6.109+/-0.54mV and 8.975+/-1.123mV and peak latencies of 10.14+/-2.743s and 10.264+/-0.95s respectively, in Cal pyramidal neurones. These evoked slow depolarising synaptic responses were suppressed by the selective muscarinic acetylcholine receptor (mAChR) antagonist atropine (5muM) suggesting that they were mediated via activation of mAChRs. In order to investigate whether GABAergic interneurones of the hippocampus are modulated by this cholinergic input, similar experiments were carried out in which whole-cell current clamp recordings were made from hippocampal intemeurones located within the stratum oriens and stratum radiatum of area CA1. (Abstract shortened by ProQuest.).

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Neurosciences.
• Colleges/Schools: College of Medical Veterinary and Life Sciences
• Supervisor's Name: Cobb, Dr. Stuart
• Date of Award: 2004
• Depositing User: Enlighten Team
• Unique ID: glathesis:2004-71181
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 10 May 2019 10:49
• Last Modified: 13 Jul 2021 09:00
• URI: https://theses.gla.ac.uk/id/eprint/71181

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