Glucuronyltransferase activity associated with hemicellulose and pectin biosynthesis in Pisum sativum

Waldron, Keith W (1984) Glucuronyltransferase activity associated with hemicellulose and pectin biosynthesis in Pisum sativum. PhD thesis, University of Glasgow.

Abstract

A particulate enzyme preparation made from 1-week-old dark-grown pea (Pisum sativum) seedlings has been shown to incorporate glucuronic acid from UDP-D-[U-14C]-glucuronic acid into a hemicellulosic product. Optimum conditions for the incorporation include the presence of Mn2+ ions between 4 and 10 mmol dm-3 and a pH of approximately 7.5. UDP-D-xylose at 1 mmol dm-3 allows incorporation to continue for at least 8 h. In its absence, the reaction stops within 30 min. Analysis of the product by partial and total acid hydrolysis, followed by paper chromatography or electrophoresis, indicates that the polysaccharide produced is a glucuronoxylan. Freezing the particulate enzyme at -20

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Plant sciences
• Date of Award: 1984
• Depositing User: Enlighten Team
• Unique ID: glathesis:1984-76522
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 19 Nov 2019 14:13
• Last Modified: 19 Nov 2019 14:13
• URI: https://theses.gla.ac.uk/id/eprint/76522