Isolation and Characterisation of E. coli xerC, a Gene Required for cer Site-Specific Recombination

Colloms, Sean David (1990) Isolation and Characterisation of E. coli xerC, a Gene Required for cer Site-Specific Recombination. PhD thesis, University of Glasgow.

Abstract

The formation of plasmid multimers by homologous recombination can greatly reduce the heritable stability of the natural multicopy plasmid ColE1. ColE1 carries a 250 bp site (cer) which, when present in direct repeat (as in a ColE1 dimer) efficiently resolves plasmid multimers to momomers. cer-mediated monomerisation is strongly correlated with an increase in plasmid stability. Current evidence suggests that ColE1 segregates randomly at cell division, and that cer-dependent monomerisation ensures stability by increasing the number of independently segregating plasmid copies (Sherratt et a 7, 1984; Summers and Sherratt, 1984).

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Genetics
• Date of Award: 1990
• Depositing User: Enlighten Team
• Unique ID: glathesis:1990-76992
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 09:24
• Last Modified: 14 Jan 2020 09:24
• URI: https://theses.gla.ac.uk/id/eprint/76992