The Regulation of Phospholipase A2 Activity in Mitogen-Stimulated Swiss 3T3 Fibroblasts

Currie, Susan (1992) The Regulation of Phospholipase A2 Activity in Mitogen-Stimulated Swiss 3T3 Fibroblasts. PhD thesis, University of Glasgow.

Abstract

A variety of agonists that stimulate inositol phospholipid turnover also activate phospholipase A2 (PLA2). This study examines the possibility that bombesin, a well-characterised calcium-mobilising peptide mitogen, may fulfil this criterion. The profile of arachidonic acid (AA) release, as well as the regulatory mechanism(s) behind bombesin-stimulated PLA2 activity, were examined. It was found that the AA released upon bombesin stimulation, was composed of two phases - an initial transient phase that is essentially independent of extracellular calcium and of PKC activation and a later, sustained phase that is absolutely dependent on extracellular calcium and shows a requirement for PKC activation. This study focusses on the first of these phases and the regulatory mechanism involved in release.

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: Biochemistry
• Date of Award: 1992
• Depositing User: Enlighten Team
• Unique ID: glathesis:1992-77322
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 14 Jan 2020 11:53
• Last Modified: 14 Jan 2020 11:53
• URI: https://theses.gla.ac.uk/id/eprint/77322