Oxidative activity and sub-cellular sorting of MSRB3

Chieruzzi, Claudia (2024) Oxidative activity and sub-cellular sorting of MSRB3. MSc(R) thesis, University of Glasgow.

Abstract

MsrB3 is one of the least well characterised methionine sulfoxide reductases and has gained interest in the field of redox biology since its discovery 20 years ago due to its association with age-related diseases, cancer, and deafness. Previous evidence suggests that MsrB3 can behave both as a methionine reductase and oxidase in vitro. The natural substrates of this enzyme in mammalian cells are unknown. Discovering the targets of MsrB3 can be technically challenging, partly because of the background stochastic oxidation of methionine from Reactive Oxygen Species that overlaps with MsrB3-induced redox changes. In addition, there is a lack of evidence on the sub-cellular targeting of endogenous MsrB3.

It was previously postulated that MsrB3 is targeted both to the ER and the mitochondria, but this study shows that it is also a found in the cytosol of mammalian cells, isolated through fractionation. Furthermore, this investigation provides additional evidence that MsrB3 could also act as an oxidase on methionine in proteins and reports a technique for studying the enzymatic oxidation of candidate MsrB3 targets by MS analysis.

• Item Type: Thesis (MSc(R))
• Qualification Level: Masters
• Subjects: Q Science > QH Natural history > QH301 Biology; Q Science > QH Natural history > QH345 Biochemistry; Q Science > QH Natural history > QH426 Genetics
• Colleges/Schools: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Supervisor's Name: Smith, Dr. Brian O. and Cao, Dr. Zhenbo
• Date of Award: 2024
• Depositing User: Theses Team
• Unique ID: glathesis:2024-84713
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 27 Nov 2024 12:14
• Last Modified: 27 Nov 2024 12:14
• Thesis DOI: 10.5525/gla.thesis.84713
• URI: https://theses.gla.ac.uk/id/eprint/84713