Development of proteomic techniques for biomarker discovery

Allingham, Heather (2012) Development of proteomic techniques for biomarker discovery. PhD thesis, University of Glasgow.

Abstract

The main aim of my research, presented here, is to develop proteomic research techniques, for their use in biomarker discovery and identification. This is broken down into three main chapters:
• Biomarker Identification in Stroke Brain guided by MALDI-imaging.
• Evaluation of the Effectiveness of Heat Treatment for Prevention of Proteomic Sample Degradation using Label Free Relative Quantitation.
• Discovery and Identification of Biomarkers for Hypertension
Within these chapters special attention is paid to sample preparation, development and assessment of new methods for biomarkers discovery and identification, the importance of experimental design and the application of relevant and useful statistical methods to enable the mining of useful information from rich datasets. The biological changes in stroke induced mouse brain tissue are studied, the prevention of degradation to tissue samples by a novel heat treatment method and changes to plasma samples from hypertensive, wild type and a congenic strain of rat are also studied. The outcomes of this work are multiple, namely:
• The identification of a possible marker for stroke from mouse brain tissue
• The effect of a new heat treatment device on proteomic data obtained from mouse brain tissue
• The novel application of a statistical analysis to a new type of dataset (LC-MS and label free quantitation of biological samples)
• The identification of possible biomarkers for hypertension in rat plasma using this method

• Item Type: Thesis (PhD)
• Qualification Level: Doctoral
• Keywords: proteomics, biomarker identification, mass spectrometry, label free quantitation, hypertension
• Subjects: Q Science > QH Natural history > QH301 Biology; Q Science > QD Chemistry
• Colleges/Schools: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Supervisor's Name: Pitt, Dr. A.R.
• Date of Award: 2012
• Depositing User: Ms Heather Allingham
• Unique ID: glathesis:2012-3145
• Copyright: Copyright of this thesis is held by the author.
• Date Deposited: 07 Feb 2012
• Last Modified: 10 Dec 2012 14:04
• URI: https://theses.gla.ac.uk/id/eprint/3145